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6201 ge 96 96 dynamic array dna binding dye sample loading reagent kit fluidigm  (fluidigm)


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    fluidigm 6201 ge 96 96 dynamic array dna binding dye sample loading reagent kit fluidigm
    6201 Ge 96 96 Dynamic Array Dna Binding Dye Sample Loading Reagent Kit Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96+96+dynamic+array+dna+binding+dye+sample+assay+loading+reagent+kit+fluidigm/DNA+Binding+Dye/pm34686326-241-12-25
    Average 95 stars, based on 265 article reviews
    6201 ge 96 96 dynamic array dna binding dye sample loading reagent kit fluidigm - by Bioz Stars, 2026-10
    95/100 stars

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    Article Title: Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4 + T Cells and Offers an Opportunity to Tackle Infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3-eFLuor450 eBioscience Cat#48-0036-42 (SK7); RRID: AB_11217677 CD4-alexaFluor700 eBioscience Cat#56-0048-82 (OKT-4); RRID: AB_657741 CD45RA-APC_H7 BD Biosciences Cat#561212 (5H9); RRID: AB_10611875 CD45RA-ECD BC Beckman coulter Cat#IM2711U (2H4LDH11LDB9); RRID: AB_10640553 CCR7-PE_Cy7 BioLegend Cat#353226 (G043H7); RRID: AB_11126145 CD27-APC Miltenyi Biotec Cat#130-093-18 (M-T271) CD27-PE BD Biosciences Cat#555441 (M-T271); RRID: AB_395834 CD95-PE Miltenyi Biotec Cat#130-092-416 (DX2); RRID: AB_871703 CD25-FITC BD Biosciences Cat#555431 (M-A251); RRID: AB_395825 CD69-FITC eBioscience Cat#11-0699-42 (FN50); RRID: AB_10853975 HLA-DR-FITC BD Biosciences Cat#555811(G46-6); RRID: AB_396145 CD25-ECD BC Beckman coulter Cat#6607112 (B1.49.9) HLA-DR_PC5.5 BC Beckman coulter Cat#B20024 Goat-anti-Mouse Alexa Fluor 647 Thermo Fisher Cat# A-21237; RRID: AB_2535806 Bacterial and Virus Strains HIV-1 BaL strain (R5) NIH AIDS reagent program Cat#510 Biological Samples Blood samples from HIV-infected individuals TRANSBioHIV study ClinicalTrials.gov Identifier: NCT01895920 Blood samples from non-infected donors Etablissement français du sang https://dondesang.efs.sante.fr/ Chemicals, Peptides, and Recombinant Proteins SuperFect transfection reagent Qiagen Cat# 301305 RPMI 1640 GlutaMAX GIBCO Cat#61870-010 RPMI Non Glucose GIBCO Cat#11879-020 Fetal Calf Serum Eurobio Cat#CVFSVF00-01 PenStrep ThermoFisher Cat# 15140-122 Lymphocyte Separation Medium (LSM) Eurobio Cat#CMSMSL01-01 Human IL-2 IS Premium grade Miltenyi Cat#130-097-748 anti-CD3 Biolegend Cat#300414 (UCHT1) 2-deoxy-glucose Agilent Cat#103020-100 (+)-Etomoxir sodium salt hydrate Sigma Cat#E1905 UK5099 Sigma Cat#PZ0160 Glucose Agilent Cat#103020-100 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose) Thermo Fisher Cat#N13195 Oligomycin Agilent Cat#103020-100 Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) Agilent Cat#103015-100 4% paraformaldehyde Thermo Fisher Cat#J61899 phytohemagglutinin-L PHA-L Roche Cat#11249738001 Human GENOMIC DNA Roche Cat#11691112001 NucleoSpin 8/96 Tissue Core kit Macherey-Nagel Cat#740453.4 RNA trace kit 96 kit Macherey-Nagel Cat#740731.4 (Continued on next page) Cell Metabolism 29, 1–16.e1–e5, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase New England Biolabs Cat#M0303L Reverse Transcription Master Mix Fluidigm Cat#100-6298 PreAmp Master Mix Fluidigm Cat#100-5580 Exonuclease I New England Biolabs Cat#M0293L SsoFast EvaGreen Supermix with Low ROX Biorad Cat#1725211 Seahorse Calibrant Solution 500mL Agilent Cat#100840-000 Seahorse XF assay medium Agilent Cat#102365-100 Sodium Pyruvate (100 mM) GIBCO Cat#11360070 Seahorse XFe96 FluxPak Agilent Cat#102416-100 Cell Tack Corning Cat#354240 LIVE/DEADTM Violet Viability dye Thermo Fisher Cat#L34955 GLUT1.RBD.FC Metafora Biosystems Cat#Glut1-M100 Critical Commercial Assays EasySep Human CD4+ T Cell Enrichment Stem Cell Technology Cat#19052 Seahorse XF Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XF Cell Mito Stress Test kit Agilent Cat#103015-100 GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit Fluidigm Cat#100-3415 HIV-1 p24 enzyme-linked immunosorbent assay XpressBio Cat#XB-1000 HIV p24 ultrasensitive digital ELISA Quanterix Cat#102215 Deposited Data Raw and analyzed data This paper http://dx.doi.org/10.17632/vfj3r27gnf.1 Experimental Models: Cell Lines HEK293T cells ECACC Cat#85120602 8E5 cells (one HIV integrated copy) Standarization ATCC Cat#CRL-8993 Oligonucleotides Delta Gene primers, Full description in Table S1 Fluidigm Custom Order U5GAG: CTT TCG CTT TCA AGT CCC TGT T (forward) David et al., 2006 Custom Order U5GAG: AGA TCC CTC AGA CCC TTT TAG TCA (reverse) David et al., 2006 Custom Order .. GCC C-(BHQ1) (probe) David et al., 2006 Custom Order Recombinant DNA Plasmid: HIV-1 NL4.3DenvDnef/GFP Amara et al., 2003 N/A Plasmid: HIV-1-DuoFluoDenv(R7GEmC) Calvanese et al., 2013; NIH AIDS Reagent Program Cat# 12595 DuoFluo (R7GEmC) Plasmind: pMD2.G fVSV-G envelope Addgene Plasmid #12259 Software and Algorithms Wave Desktop Agilent / Seahorse Bioscience https://www.agilent.com/en/products/cell- analysis/software-download-for-wavedesktop Prism Graphpad https://www.graphpad.com/scientific- software/prism/ Normfinder algorithm Andersen et al., 2004 Aarhus University Hospital, Denmark SigmaPlot Systat Software http://www.sigmaplot.co.uk/products/ sigmaplot/sigmaplot-details.php R studio R Development Core Team http://www.R-project.org lme4 v1.1-18-1 Bates et al. (2015) https://cran.r-project.org/web/packages/ lme4/index.html MASS v7.3-51 Venables and Ripley (2002) https://cran.r-project.org/web/packages/ MASS/index.html e2 Cell Metabolism 29, 1–16.e1–e5, April 2, 2019

    XF Assay:

    Article Title: Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4 + T Cells and Offers an Opportunity to Tackle Infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3-eFLuor450 eBioscience Cat#48-0036-42 (SK7); RRID: AB_11217677 CD4-alexaFluor700 eBioscience Cat#56-0048-82 (OKT-4); RRID: AB_657741 CD45RA-APC_H7 BD Biosciences Cat#561212 (5H9); RRID: AB_10611875 CD45RA-ECD BC Beckman coulter Cat#IM2711U (2H4LDH11LDB9); RRID: AB_10640553 CCR7-PE_Cy7 BioLegend Cat#353226 (G043H7); RRID: AB_11126145 CD27-APC Miltenyi Biotec Cat#130-093-18 (M-T271) CD27-PE BD Biosciences Cat#555441 (M-T271); RRID: AB_395834 CD95-PE Miltenyi Biotec Cat#130-092-416 (DX2); RRID: AB_871703 CD25-FITC BD Biosciences Cat#555431 (M-A251); RRID: AB_395825 CD69-FITC eBioscience Cat#11-0699-42 (FN50); RRID: AB_10853975 HLA-DR-FITC BD Biosciences Cat#555811(G46-6); RRID: AB_396145 CD25-ECD BC Beckman coulter Cat#6607112 (B1.49.9) HLA-DR_PC5.5 BC Beckman coulter Cat#B20024 Goat-anti-Mouse Alexa Fluor 647 Thermo Fisher Cat# A-21237; RRID: AB_2535806 Bacterial and Virus Strains HIV-1 BaL strain (R5) NIH AIDS reagent program Cat#510 Biological Samples Blood samples from HIV-infected individuals TRANSBioHIV study ClinicalTrials.gov Identifier: NCT01895920 Blood samples from non-infected donors Etablissement français du sang https://dondesang.efs.sante.fr/ Chemicals, Peptides, and Recombinant Proteins SuperFect transfection reagent Qiagen Cat# 301305 RPMI 1640 GlutaMAX GIBCO Cat#61870-010 RPMI Non Glucose GIBCO Cat#11879-020 Fetal Calf Serum Eurobio Cat#CVFSVF00-01 PenStrep ThermoFisher Cat# 15140-122 Lymphocyte Separation Medium (LSM) Eurobio Cat#CMSMSL01-01 Human IL-2 IS Premium grade Miltenyi Cat#130-097-748 anti-CD3 Biolegend Cat#300414 (UCHT1) 2-deoxy-glucose Agilent Cat#103020-100 (+)-Etomoxir sodium salt hydrate Sigma Cat#E1905 UK5099 Sigma Cat#PZ0160 Glucose Agilent Cat#103020-100 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose) Thermo Fisher Cat#N13195 Oligomycin Agilent Cat#103020-100 Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) Agilent Cat#103015-100 4% paraformaldehyde Thermo Fisher Cat#J61899 phytohemagglutinin-L PHA-L Roche Cat#11249738001 Human GENOMIC DNA Roche Cat#11691112001 NucleoSpin 8/96 Tissue Core kit Macherey-Nagel Cat#740453.4 RNA trace kit 96 kit Macherey-Nagel Cat#740731.4 (Continued on next page) Cell Metabolism 29, 1–16.e1–e5, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase New England Biolabs Cat#M0303L Reverse Transcription Master Mix Fluidigm Cat#100-6298 PreAmp Master Mix Fluidigm Cat#100-5580 Exonuclease I New England Biolabs Cat#M0293L SsoFast EvaGreen Supermix with Low ROX Biorad Cat#1725211 Seahorse Calibrant Solution 500mL Agilent Cat#100840-000 Seahorse XF assay medium Agilent Cat#102365-100 Sodium Pyruvate (100 mM) GIBCO Cat#11360070 Seahorse XFe96 FluxPak Agilent Cat#102416-100 Cell Tack Corning Cat#354240 LIVE/DEADTM Violet Viability dye Thermo Fisher Cat#L34955 GLUT1.RBD.FC Metafora Biosystems Cat#Glut1-M100 Critical Commercial Assays EasySep Human CD4+ T Cell Enrichment Stem Cell Technology Cat#19052 Seahorse XF Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XF Cell Mito Stress Test kit Agilent Cat#103015-100 GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit Fluidigm Cat#100-3415 HIV-1 p24 enzyme-linked immunosorbent assay XpressBio Cat#XB-1000 HIV p24 ultrasensitive digital ELISA Quanterix Cat#102215 Deposited Data Raw and analyzed data This paper http://dx.doi.org/10.17632/vfj3r27gnf.1 Experimental Models: Cell Lines HEK293T cells ECACC Cat#85120602 8E5 cells (one HIV integrated copy) Standarization ATCC Cat#CRL-8993 Oligonucleotides Delta Gene primers, Full description in Table S1 Fluidigm Custom Order U5GAG: CTT TCG CTT TCA AGT CCC TGT T (forward) David et al., 2006 Custom Order U5GAG: AGA TCC CTC AGA CCC TTT TAG TCA (reverse) David et al., 2006 Custom Order .. GCC C-(BHQ1) (probe) David et al., 2006 Custom Order Recombinant DNA Plasmid: HIV-1 NL4.3DenvDnef/GFP Amara et al., 2003 N/A Plasmid: HIV-1-DuoFluoDenv(R7GEmC) Calvanese et al., 2013; NIH AIDS Reagent Program Cat# 12595 DuoFluo (R7GEmC) Plasmind: pMD2.G fVSV-G envelope Addgene Plasmid #12259 Software and Algorithms Wave Desktop Agilent / Seahorse Bioscience https://www.agilent.com/en/products/cell- analysis/software-download-for-wavedesktop Prism Graphpad https://www.graphpad.com/scientific- software/prism/ Normfinder algorithm Andersen et al., 2004 Aarhus University Hospital, Denmark SigmaPlot Systat Software http://www.sigmaplot.co.uk/products/ sigmaplot/sigmaplot-details.php R studio R Development Core Team http://www.R-project.org lme4 v1.1-18-1 Bates et al. (2015) https://cran.r-project.org/web/packages/ lme4/index.html MASS v7.3-51 Venables and Ripley (2002) https://cran.r-project.org/web/packages/ MASS/index.html e2 Cell Metabolism 29, 1–16.e1–e5, April 2, 2019

    Binding Assay:

    Article Title: Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4 + T Cells and Offers an Opportunity to Tackle Infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3-eFLuor450 eBioscience Cat#48-0036-42 (SK7); RRID: AB_11217677 CD4-alexaFluor700 eBioscience Cat#56-0048-82 (OKT-4); RRID: AB_657741 CD45RA-APC_H7 BD Biosciences Cat#561212 (5H9); RRID: AB_10611875 CD45RA-ECD BC Beckman coulter Cat#IM2711U (2H4LDH11LDB9); RRID: AB_10640553 CCR7-PE_Cy7 BioLegend Cat#353226 (G043H7); RRID: AB_11126145 CD27-APC Miltenyi Biotec Cat#130-093-18 (M-T271) CD27-PE BD Biosciences Cat#555441 (M-T271); RRID: AB_395834 CD95-PE Miltenyi Biotec Cat#130-092-416 (DX2); RRID: AB_871703 CD25-FITC BD Biosciences Cat#555431 (M-A251); RRID: AB_395825 CD69-FITC eBioscience Cat#11-0699-42 (FN50); RRID: AB_10853975 HLA-DR-FITC BD Biosciences Cat#555811(G46-6); RRID: AB_396145 CD25-ECD BC Beckman coulter Cat#6607112 (B1.49.9) HLA-DR_PC5.5 BC Beckman coulter Cat#B20024 Goat-anti-Mouse Alexa Fluor 647 Thermo Fisher Cat# A-21237; RRID: AB_2535806 Bacterial and Virus Strains HIV-1 BaL strain (R5) NIH AIDS reagent program Cat#510 Biological Samples Blood samples from HIV-infected individuals TRANSBioHIV study ClinicalTrials.gov Identifier: NCT01895920 Blood samples from non-infected donors Etablissement français du sang https://dondesang.efs.sante.fr/ Chemicals, Peptides, and Recombinant Proteins SuperFect transfection reagent Qiagen Cat# 301305 RPMI 1640 GlutaMAX GIBCO Cat#61870-010 RPMI Non Glucose GIBCO Cat#11879-020 Fetal Calf Serum Eurobio Cat#CVFSVF00-01 PenStrep ThermoFisher Cat# 15140-122 Lymphocyte Separation Medium (LSM) Eurobio Cat#CMSMSL01-01 Human IL-2 IS Premium grade Miltenyi Cat#130-097-748 anti-CD3 Biolegend Cat#300414 (UCHT1) 2-deoxy-glucose Agilent Cat#103020-100 (+)-Etomoxir sodium salt hydrate Sigma Cat#E1905 UK5099 Sigma Cat#PZ0160 Glucose Agilent Cat#103020-100 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose) Thermo Fisher Cat#N13195 Oligomycin Agilent Cat#103020-100 Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) Agilent Cat#103015-100 4% paraformaldehyde Thermo Fisher Cat#J61899 phytohemagglutinin-L PHA-L Roche Cat#11249738001 Human GENOMIC DNA Roche Cat#11691112001 NucleoSpin 8/96 Tissue Core kit Macherey-Nagel Cat#740453.4 RNA trace kit 96 kit Macherey-Nagel Cat#740731.4 (Continued on next page) Cell Metabolism 29, 1–16.e1–e5, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase New England Biolabs Cat#M0303L Reverse Transcription Master Mix Fluidigm Cat#100-6298 PreAmp Master Mix Fluidigm Cat#100-5580 Exonuclease I New England Biolabs Cat#M0293L SsoFast EvaGreen Supermix with Low ROX Biorad Cat#1725211 Seahorse Calibrant Solution 500mL Agilent Cat#100840-000 Seahorse XF assay medium Agilent Cat#102365-100 Sodium Pyruvate (100 mM) GIBCO Cat#11360070 Seahorse XFe96 FluxPak Agilent Cat#102416-100 Cell Tack Corning Cat#354240 LIVE/DEADTM Violet Viability dye Thermo Fisher Cat#L34955 GLUT1.RBD.FC Metafora Biosystems Cat#Glut1-M100 Critical Commercial Assays EasySep Human CD4+ T Cell Enrichment Stem Cell Technology Cat#19052 Seahorse XF Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XF Cell Mito Stress Test kit Agilent Cat#103015-100 GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit Fluidigm Cat#100-3415 HIV-1 p24 enzyme-linked immunosorbent assay XpressBio Cat#XB-1000 HIV p24 ultrasensitive digital ELISA Quanterix Cat#102215 Deposited Data Raw and analyzed data This paper http://dx.doi.org/10.17632/vfj3r27gnf.1 Experimental Models: Cell Lines HEK293T cells ECACC Cat#85120602 8E5 cells (one HIV integrated copy) Standarization ATCC Cat#CRL-8993 Oligonucleotides Delta Gene primers, Full description in Table S1 Fluidigm Custom Order U5GAG: CTT TCG CTT TCA AGT CCC TGT T (forward) David et al., 2006 Custom Order U5GAG: AGA TCC CTC AGA CCC TTT TAG TCA (reverse) David et al., 2006 Custom Order .. GCC C-(BHQ1) (probe) David et al., 2006 Custom Order Recombinant DNA Plasmid: HIV-1 NL4.3DenvDnef/GFP Amara et al., 2003 N/A Plasmid: HIV-1-DuoFluoDenv(R7GEmC) Calvanese et al., 2013; NIH AIDS Reagent Program Cat# 12595 DuoFluo (R7GEmC) Plasmind: pMD2.G fVSV-G envelope Addgene Plasmid #12259 Software and Algorithms Wave Desktop Agilent / Seahorse Bioscience https://www.agilent.com/en/products/cell- analysis/software-download-for-wavedesktop Prism Graphpad https://www.graphpad.com/scientific- software/prism/ Normfinder algorithm Andersen et al., 2004 Aarhus University Hospital, Denmark SigmaPlot Systat Software http://www.sigmaplot.co.uk/products/ sigmaplot/sigmaplot-details.php R studio R Development Core Team http://www.R-project.org lme4 v1.1-18-1 Bates et al. (2015) https://cran.r-project.org/web/packages/ lme4/index.html MASS v7.3-51 Venables and Ripley (2002) https://cran.r-project.org/web/packages/ MASS/index.html e2 Cell Metabolism 29, 1–16.e1–e5, April 2, 2019

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4 + T Cells and Offers an Opportunity to Tackle Infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3-eFLuor450 eBioscience Cat#48-0036-42 (SK7); RRID: AB_11217677 CD4-alexaFluor700 eBioscience Cat#56-0048-82 (OKT-4); RRID: AB_657741 CD45RA-APC_H7 BD Biosciences Cat#561212 (5H9); RRID: AB_10611875 CD45RA-ECD BC Beckman coulter Cat#IM2711U (2H4LDH11LDB9); RRID: AB_10640553 CCR7-PE_Cy7 BioLegend Cat#353226 (G043H7); RRID: AB_11126145 CD27-APC Miltenyi Biotec Cat#130-093-18 (M-T271) CD27-PE BD Biosciences Cat#555441 (M-T271); RRID: AB_395834 CD95-PE Miltenyi Biotec Cat#130-092-416 (DX2); RRID: AB_871703 CD25-FITC BD Biosciences Cat#555431 (M-A251); RRID: AB_395825 CD69-FITC eBioscience Cat#11-0699-42 (FN50); RRID: AB_10853975 HLA-DR-FITC BD Biosciences Cat#555811(G46-6); RRID: AB_396145 CD25-ECD BC Beckman coulter Cat#6607112 (B1.49.9) HLA-DR_PC5.5 BC Beckman coulter Cat#B20024 Goat-anti-Mouse Alexa Fluor 647 Thermo Fisher Cat# A-21237; RRID: AB_2535806 Bacterial and Virus Strains HIV-1 BaL strain (R5) NIH AIDS reagent program Cat#510 Biological Samples Blood samples from HIV-infected individuals TRANSBioHIV study ClinicalTrials.gov Identifier: NCT01895920 Blood samples from non-infected donors Etablissement français du sang https://dondesang.efs.sante.fr/ Chemicals, Peptides, and Recombinant Proteins SuperFect transfection reagent Qiagen Cat# 301305 RPMI 1640 GlutaMAX GIBCO Cat#61870-010 RPMI Non Glucose GIBCO Cat#11879-020 Fetal Calf Serum Eurobio Cat#CVFSVF00-01 PenStrep ThermoFisher Cat# 15140-122 Lymphocyte Separation Medium (LSM) Eurobio Cat#CMSMSL01-01 Human IL-2 IS Premium grade Miltenyi Cat#130-097-748 anti-CD3 Biolegend Cat#300414 (UCHT1) 2-deoxy-glucose Agilent Cat#103020-100 (+)-Etomoxir sodium salt hydrate Sigma Cat#E1905 UK5099 Sigma Cat#PZ0160 Glucose Agilent Cat#103020-100 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose) Thermo Fisher Cat#N13195 Oligomycin Agilent Cat#103020-100 Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) Agilent Cat#103015-100 4% paraformaldehyde Thermo Fisher Cat#J61899 phytohemagglutinin-L PHA-L Roche Cat#11249738001 Human GENOMIC DNA Roche Cat#11691112001 NucleoSpin 8/96 Tissue Core kit Macherey-Nagel Cat#740453.4 RNA trace kit 96 kit Macherey-Nagel Cat#740731.4 (Continued on next page) Cell Metabolism 29, 1–16.e1–e5, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase New England Biolabs Cat#M0303L Reverse Transcription Master Mix Fluidigm Cat#100-6298 PreAmp Master Mix Fluidigm Cat#100-5580 Exonuclease I New England Biolabs Cat#M0293L SsoFast EvaGreen Supermix with Low ROX Biorad Cat#1725211 Seahorse Calibrant Solution 500mL Agilent Cat#100840-000 Seahorse XF assay medium Agilent Cat#102365-100 Sodium Pyruvate (100 mM) GIBCO Cat#11360070 Seahorse XFe96 FluxPak Agilent Cat#102416-100 Cell Tack Corning Cat#354240 LIVE/DEADTM Violet Viability dye Thermo Fisher Cat#L34955 GLUT1.RBD.FC Metafora Biosystems Cat#Glut1-M100 Critical Commercial Assays EasySep Human CD4+ T Cell Enrichment Stem Cell Technology Cat#19052 Seahorse XF Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XF Cell Mito Stress Test kit Agilent Cat#103015-100 GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit Fluidigm Cat#100-3415 HIV-1 p24 enzyme-linked immunosorbent assay XpressBio Cat#XB-1000 HIV p24 ultrasensitive digital ELISA Quanterix Cat#102215 Deposited Data Raw and analyzed data This paper http://dx.doi.org/10.17632/vfj3r27gnf.1 Experimental Models: Cell Lines HEK293T cells ECACC Cat#85120602 8E5 cells (one HIV integrated copy) Standarization ATCC Cat#CRL-8993 Oligonucleotides Delta Gene primers, Full description in Table S1 Fluidigm Custom Order U5GAG: CTT TCG CTT TCA AGT CCC TGT T (forward) David et al., 2006 Custom Order U5GAG: AGA TCC CTC AGA CCC TTT TAG TCA (reverse) David et al., 2006 Custom Order .. GCC C-(BHQ1) (probe) David et al., 2006 Custom Order Recombinant DNA Plasmid: HIV-1 NL4.3DenvDnef/GFP Amara et al., 2003 N/A Plasmid: HIV-1-DuoFluoDenv(R7GEmC) Calvanese et al., 2013; NIH AIDS Reagent Program Cat# 12595 DuoFluo (R7GEmC) Plasmind: pMD2.G fVSV-G envelope Addgene Plasmid #12259 Software and Algorithms Wave Desktop Agilent / Seahorse Bioscience https://www.agilent.com/en/products/cell- analysis/software-download-for-wavedesktop Prism Graphpad https://www.graphpad.com/scientific- software/prism/ Normfinder algorithm Andersen et al., 2004 Aarhus University Hospital, Denmark SigmaPlot Systat Software http://www.sigmaplot.co.uk/products/ sigmaplot/sigmaplot-details.php R studio R Development Core Team http://www.R-project.org lme4 v1.1-18-1 Bates et al. (2015) https://cran.r-project.org/web/packages/ lme4/index.html MASS v7.3-51 Venables and Ripley (2002) https://cran.r-project.org/web/packages/ MASS/index.html e2 Cell Metabolism 29, 1–16.e1–e5, April 2, 2019

    Countercurrent Chromatography:

    Article Title: Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4 + T Cells and Offers an Opportunity to Tackle Infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3-eFLuor450 eBioscience Cat#48-0036-42 (SK7); RRID: AB_11217677 CD4-alexaFluor700 eBioscience Cat#56-0048-82 (OKT-4); RRID: AB_657741 CD45RA-APC_H7 BD Biosciences Cat#561212 (5H9); RRID: AB_10611875 CD45RA-ECD BC Beckman coulter Cat#IM2711U (2H4LDH11LDB9); RRID: AB_10640553 CCR7-PE_Cy7 BioLegend Cat#353226 (G043H7); RRID: AB_11126145 CD27-APC Miltenyi Biotec Cat#130-093-18 (M-T271) CD27-PE BD Biosciences Cat#555441 (M-T271); RRID: AB_395834 CD95-PE Miltenyi Biotec Cat#130-092-416 (DX2); RRID: AB_871703 CD25-FITC BD Biosciences Cat#555431 (M-A251); RRID: AB_395825 CD69-FITC eBioscience Cat#11-0699-42 (FN50); RRID: AB_10853975 HLA-DR-FITC BD Biosciences Cat#555811(G46-6); RRID: AB_396145 CD25-ECD BC Beckman coulter Cat#6607112 (B1.49.9) HLA-DR_PC5.5 BC Beckman coulter Cat#B20024 Goat-anti-Mouse Alexa Fluor 647 Thermo Fisher Cat# A-21237; RRID: AB_2535806 Bacterial and Virus Strains HIV-1 BaL strain (R5) NIH AIDS reagent program Cat#510 Biological Samples Blood samples from HIV-infected individuals TRANSBioHIV study ClinicalTrials.gov Identifier: NCT01895920 Blood samples from non-infected donors Etablissement français du sang https://dondesang.efs.sante.fr/ Chemicals, Peptides, and Recombinant Proteins SuperFect transfection reagent Qiagen Cat# 301305 RPMI 1640 GlutaMAX GIBCO Cat#61870-010 RPMI Non Glucose GIBCO Cat#11879-020 Fetal Calf Serum Eurobio Cat#CVFSVF00-01 PenStrep ThermoFisher Cat# 15140-122 Lymphocyte Separation Medium (LSM) Eurobio Cat#CMSMSL01-01 Human IL-2 IS Premium grade Miltenyi Cat#130-097-748 anti-CD3 Biolegend Cat#300414 (UCHT1) 2-deoxy-glucose Agilent Cat#103020-100 (+)-Etomoxir sodium salt hydrate Sigma Cat#E1905 UK5099 Sigma Cat#PZ0160 Glucose Agilent Cat#103020-100 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose) Thermo Fisher Cat#N13195 Oligomycin Agilent Cat#103020-100 Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) Agilent Cat#103015-100 4% paraformaldehyde Thermo Fisher Cat#J61899 phytohemagglutinin-L PHA-L Roche Cat#11249738001 Human GENOMIC DNA Roche Cat#11691112001 NucleoSpin 8/96 Tissue Core kit Macherey-Nagel Cat#740453.4 RNA trace kit 96 kit Macherey-Nagel Cat#740731.4 (Continued on next page) Cell Metabolism 29, 1–16.e1–e5, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase New England Biolabs Cat#M0303L Reverse Transcription Master Mix Fluidigm Cat#100-6298 PreAmp Master Mix Fluidigm Cat#100-5580 Exonuclease I New England Biolabs Cat#M0293L SsoFast EvaGreen Supermix with Low ROX Biorad Cat#1725211 Seahorse Calibrant Solution 500mL Agilent Cat#100840-000 Seahorse XF assay medium Agilent Cat#102365-100 Sodium Pyruvate (100 mM) GIBCO Cat#11360070 Seahorse XFe96 FluxPak Agilent Cat#102416-100 Cell Tack Corning Cat#354240 LIVE/DEADTM Violet Viability dye Thermo Fisher Cat#L34955 GLUT1.RBD.FC Metafora Biosystems Cat#Glut1-M100 Critical Commercial Assays EasySep Human CD4+ T Cell Enrichment Stem Cell Technology Cat#19052 Seahorse XF Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XF Cell Mito Stress Test kit Agilent Cat#103015-100 GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit Fluidigm Cat#100-3415 HIV-1 p24 enzyme-linked immunosorbent assay XpressBio Cat#XB-1000 HIV p24 ultrasensitive digital ELISA Quanterix Cat#102215 Deposited Data Raw and analyzed data This paper http://dx.doi.org/10.17632/vfj3r27gnf.1 Experimental Models: Cell Lines HEK293T cells ECACC Cat#85120602 8E5 cells (one HIV integrated copy) Standarization ATCC Cat#CRL-8993 Oligonucleotides Delta Gene primers, Full description in Table S1 Fluidigm Custom Order U5GAG: CTT TCG CTT TCA AGT CCC TGT T (forward) David et al., 2006 Custom Order U5GAG: AGA TCC CTC AGA CCC TTT TAG TCA (reverse) David et al., 2006 Custom Order .. GCC C-(BHQ1) (probe) David et al., 2006 Custom Order Recombinant DNA Plasmid: HIV-1 NL4.3DenvDnef/GFP Amara et al., 2003 N/A Plasmid: HIV-1-DuoFluoDenv(R7GEmC) Calvanese et al., 2013; NIH AIDS Reagent Program Cat# 12595 DuoFluo (R7GEmC) Plasmind: pMD2.G fVSV-G envelope Addgene Plasmid #12259 Software and Algorithms Wave Desktop Agilent / Seahorse Bioscience https://www.agilent.com/en/products/cell- analysis/software-download-for-wavedesktop Prism Graphpad https://www.graphpad.com/scientific- software/prism/ Normfinder algorithm Andersen et al., 2004 Aarhus University Hospital, Denmark SigmaPlot Systat Software http://www.sigmaplot.co.uk/products/ sigmaplot/sigmaplot-details.php R studio R Development Core Team http://www.R-project.org lme4 v1.1-18-1 Bates et al. (2015) https://cran.r-project.org/web/packages/ lme4/index.html MASS v7.3-51 Venables and Ripley (2002) https://cran.r-project.org/web/packages/ MASS/index.html e2 Cell Metabolism 29, 1–16.e1–e5, April 2, 2019



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